Incubation oven (catalog #166-0501EDU) 1 ❏
Microcentrifuge (catalog #166-0602EDU) 1 ❏
Mini centrifuge (catalog #166-0603EDU) 1 ❏
Microtube centrifuge tube racks
(catalog #166-0481EDU) 8 ❏
Storage boxes (catalog #166-0482EDU) ❏
Refresh Kit Components
Catalog # Product Description
166-0015EDU Lyophilized components refill package, includes ampicillin,
arabinose, LB nutrient broth tablet, lysozyme
166-0406EDU Arabinose, 600 mg, lyophilized
166-0407EDU Ampicillin, 30 mg, lyophilized
166-0600EDU LB nutrient agar powder, 20 g, makes forty, 60 mm agar plates
166-0472EDU LB nutrient agar powder, 500 g, makes one thousand,
60 mm agar plates
166-0016EDU Chromatography buffers refill package, includes binding buffer,
column equilibration buffer, column wash buffer, elution buffer (TE)
166-0413EDU HIC chromatography columns and caps, 8 each
166-0471EDU Inoculation loops, 10 ml, sterile, 80
166-0474EDU Disposable plastic transfer pipets, sterile, 500
166-0480EDU Disposable plastic transfer pipets, nonsterile, 500
166-0476EDU Cell culture tubes, 17 x 100 mm, 14 ml, sterile, 25
223-9750EDU Clear polystyrene tubes, 13 x 100 mm, 9 ml, 1,000
166-0470EDU Petri dishes, 60 mm, sterile, 500
223-9430EDU EZ Micro
TM
Test Tubes, 2.0 ml, natural, 500
166-0479EDU Jellyfish foam floating racks, 8 racks with 12 microcentrifuge tube
wells
Safety Issues
The
Escherichia coli
bacteria HB101 K-12 strain contained in this kit is not a pathogenic organ-
ism like the
E. coli
strain O157 H7 that has sometimes been implicated in food poisoning.
HB101 K-12 has been genetically modified to prevent its growth unless grown on an enriched
medium. However, handling of the
E. coli
K-12 strain requires the use of standard
Microbiological Practices. These practices include, but are not limited to, the following. Work
surfaces are decontaminated once a day and after any spill of viable material. All contaminated
liquid or solid wastes are decontaminated before disposal. All persons must wash their hands:
(i) after they handle material containing bacteria, and (ii) before exiting the laboratory. All pro-
cedures are performed carefully to minimize the creation of aerosols. Mechanical pipeting
devices are used, mouth pipetting is prohibited; eating, drinking, smoking, and applying cos-
metics are not permitted in the work area; wearing protective eyewear and gloves is strong-
ly recommended.
If an autoclave is not available, all solutions and components (loops and pipets) that have
come in contact with bacteria can be placed in a fresh 10% bleach solution for at least
20 min for sterilization. A shallow pan of this solution should be placed at every lab station. No
matter what you choose, all used loops and pipets should be collected for sterilization. Sterilize
petri dishes by covering the agar with 10% bleach solution. Let the plate stand for
1 hr or more, and then pour excess plate liquid down the drain. Once sterilized, the agar
plates can be double bagged and treated as normal trash. Safety glasses are recommended
when using bleach solutions.
3